IDENTIFICATION OF THE REPLICATION REGION OF LACTOBACILLUS-ACIDOPHILUS PLASMID PLA103

被引：0
|
作者：
KANATANI, K [1 ]
TAHARA, T [1 ]
OSHIMURA, M [1 ]
SANO, K [1 ]
UMEZAWA, C [1 ]
机构：
[1] KOBE GAKUIN UNIV,SCH PHARM,BIOCHEM LAB,NISHI KU,KOBE 673,JAPAN
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 1-2期
关键词：
LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS PLASMID; PLASMID REPLICATION; PLASMID VECTOR;
D O I：
10.1016/0378-1097(95)00348-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The structure of the region necessary for replication of the plasmid pLA103 from Lactobacillus acidophilus TK8912 has been characterized. Sequence analysis revealed that the replication region contained an open reading frame (OrfA) encoding a 282-amino acid peptide preceded by a 22-bp tandem repeat sequence region. The predicted OrfA protein showed homology to the replication protein of a plasmid from Pediococcus halophilus. The plasmid containing the repeat sequence region preceding OrfA was able to replicate in the Lactobacillus host when provided with OrfA in tl-ans, suggesting that the repeat sequence region contains the origin sequence essential for the pLA103 replication.
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页码：127 / 130
页数：4
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