ISOENZYME ANALYSIS OF HAMMONDIA-HAMMONDI AND TOXOPLASMA-GONDII SPOROZOITES

被引：7
|
作者：
DARDE, ML
RIAHI, H
BOUTEILLE, B
PESTREALEXANDRE, M
机构：
[1] Service de Parasitologie-Mycologie, CHU Dupuytren
来源：
JOURNAL OF PARASITOLOGY | 1992年 / 78卷 / 04期
关键词：
D O I：
10.2307/3283552
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species.
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页码：731 / 734
页数：4
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