IMMOBILIZATION OF BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1 FOR OLIGOSACCHARIDES SYNTHESIS

被引：17
|
作者：
BERGER, JL
LEE, BH
LACROIX, C
机构：
[1] MCGILL UNIV,DEPT FOOD SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA
[2] AGR CANADA,FRDC,ST HYACINTHE,PQ J2S 8E3,CANADA
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 08期
关键词：
D O I：
10.1007/BF00152452
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
beta-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was developed using glutaraldehyde co-crosslinking of beta-galactosidases with bovine serum albumin, followed by entrapment in agarose beads.
引用
收藏
页码：601 / 606
页数：6
相关论文
共 50 条
  • [21] CARBOXYPEPTIDASE TAQ, A THERMOSTABLE ZINC ENZYME, FROM THERMUS-AQUATICUS YT-1 - MOLECULAR-CLONING, SEQUENCING, AND EXPRESSION OF THE ENCODING GENE IN ESCHERICHIA-COLI
    LEE, SH
    TAGUCHI, H
    YOSHIMURA, E
    MINAGAWA, E
    KAMINOGAWA, S
    OHTA, T
    MATSUZAWA, H
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (08) : 1490 - 1495
  • [22] P25, an AT-rich DNA-binding-protein from Thermus aquaticus YT-1
    Du, X
    Cain, WJ
    Pene, JJ
    FASEB JOURNAL, 1997, 11 (09): : A1368 - A1368
  • [23] NUCLEOTIDE-SEQUENCE AND CHARACTERISTICS OF THE GENE FOR L-LACTATE DEHYDROGENASE OF THERMUS-AQUATICUS YT-1 AND THE DEDUCED AMINO-ACID-SEQUENCE OF THE ENZYME
    ONO, M
    MATSUZAWA, H
    OHTA, T
    JOURNAL OF BIOCHEMISTRY, 1990, 107 (01): : 21 - 26
  • [24] ISOLATION AND PROPERTIES OF DNA-POLYMERASE FROM EXTREMELY THERMOPHILIC BACTERIUM THERMUS-AQUATICUS YT1
    KALEDIN, AS
    SLYUSARENKO, AG
    GORODETSKII, SI
    BIOCHEMISTRY-MOSCOW, 1980, 45 (04) : 494 - 501
  • [25] Crystallization and preliminary crystallographic analysis of an NADH oxidase that functions in peroxide reduction in Thermus aquaticus YT-1
    Mac Sweeney, A
    D'Arcy, A
    Higgins, TM
    Mayhew, SG
    Toomey, D
    Walsh, MA
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 297 - 298
  • [26] NUCLEOTIDE-SEQUENCE OF THE GENE FOR AQUALYSIN-I (A THERMOPHILIC ALKALINE SERINE PROTEASE) OF THERMUS-AQUATICUS YT-1 AND CHARACTERISTICS OF THE DEDUCED PRIMARY STRUCTURE OF THE ENZYME
    KWON, ST
    TERADA, I
    MATSUZAWA, H
    OHTA, T
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (03): : 491 - 497
  • [27] The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities
    Skowron, Piotr M.
    Anton, Brian P.
    Czajkowska, Edyta
    Zebrowska, Joanna
    Sulecka, Ewa
    Krefft, Daria
    Jezewska-Frackowiak, Joanna
    Zolnierkiewicz, Olga
    Witkowska, Malgorzata
    Morgan, Richard D.
    Wilson, Geoffrey G.
    Fomenkov, Alexey
    Roberts, Richard J.
    Zylicz-Stachula, Agnieszka
    NUCLEIC ACIDS RESEARCH, 2017, 45 (15) : 9005 - 9018
  • [28] Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system
    Toomey, D.
    Mayhew, S. G.
    European Journal of Biochemistry, 251 (03):
  • [29] Stability of thermostable enzyme, aqualysin I;: a subtilisin-type serine protease from Thermus aquaticus YT-1
    Tanaka, T
    Matsuzawa, H
    Ohta, T
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (09) : 1806 - 1808
  • [30] Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system
    Toomey, D
    Mayhew, SG
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 251 (03): : 935 - 945
12345