IMMOBILIZATION OF BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1 FOR OLIGOSACCHARIDES SYNTHESIS

被引：17
|
作者：
BERGER, JL
LEE, BH
LACROIX, C
机构：
[1] MCGILL UNIV,DEPT FOOD SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA
[2] AGR CANADA,FRDC,ST HYACINTHE,PQ J2S 8E3,CANADA
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 08期
关键词：
D O I：
10.1007/BF00152452
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
beta-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was developed using glutaraldehyde co-crosslinking of beta-galactosidases with bovine serum albumin, followed by entrapment in agarose beads.
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页码：601 / 606
页数：6
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