INFLUENCE OF THE PH OF THE EXTRACTION MEDIUM ON THE COMPOSITION OF BIRCH (BETULA-VERRUCOSA) POLLEN EXTRACTS

被引：18

作者：

CADOT, P ^{[1
]}

LEJOLY, M ^{[1
]}

VANHOEYVELD, EW ^{[1
]}

STEVENS, EAM ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,UNIV ZIEKENHUIS ST RAFAEL,CLIN IMMUNOL LAB,B-3000 LOUVAIN,BELGIUM

来源：

ALLERGY | 1995年 / 50卷 / 05期

关键词：

ALLERGENS; BIRCH POLLEN; EXTRACTION; ISOELECTRIC FOCUSING; IMMUNOBLOTTING; PH; SDS-PAGE;

D O I：

10.1111/j.1398-9995.1995.tb01173.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Extracts from birch (Betula verrucosa) pollen were prepared at different pH, with constant pH monitoring and adjustment to preset values in the range 5.5-8.5. The total protein content of these extracts was directly correlated with the pH. Coomassie brilliant blue-stained isoelectric focusing and SDS-PAGE gels and immunoblot analysis demonstrated qualitative differences: some proteins were lost while others appeared when pH was changed. At pH 8.5, formerly unknown birch pollen allergens were detected with pI 9, 9.10, and 9.30 by about 30% of birch pollen-sensitive sera. Birch pollen extracts prepared at a pH close to neutrality, namely, 6.5 and 7.5, showed the greatest protein and different allergen diversity. Thus, extraction pH values are necessary to analyze the whole pattern of allergenic components in an extract.

引用

页码：431 / 437

页数：7

共 50 条

[41] The effect of extraction process on polyphenol content, triterpene composition and bioactivity of yellow birch (Betula alleghaniensis Britton) extracts
Diouf, Papa Niokhor
Stevanovic, Tatjana
Boutin, Yvan
INDUSTRIAL CROPS AND PRODUCTS, 2009, 30 (02) : 297 - 303
[42] STUDIES ON COMPONENTS IN WOOD .7. IDENTIFICATION OF TOTAL FATTY-ACIDS IN BIRCH (BETULA-VERRUCOSA) BY GAS-CHROMATOGRAPHY - MASS-SPECTROMETRY
EKMAN, R
PENSAR, G
FINSKA KEMISTSAMFUNDETS MEDDELANDEN, 1973, 82 (04): : 105 - 113
[43] THE INFLUENCE OF HORMONES COMPOSITION IN THE PRECULTIVATION MEDIUM ON REGENERATION OF CALLUS CULTURES OF SILVER BIRCH (BETULA PENDULA ROTH.)
Konstantinov, Andrei V.
MODERN PHYTOMORPHOLOGY, 2013, 4 : 241 - 244
[44] Studies of the chemical composition of birch bark extracts (Cortex betula) from the Betulaceae family
Abyshev A.Z.
Agaev É.M.
Guseinov A.B.
Pharmaceutical Chemistry Journal, 2007, 41 (8) : 419 - 423
[45] Variation of chemical composition of the lipophilic extracts from yellow birch (Betula alleghaniensis) foliage
Lavoie, JM
Stevanovic, T
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (12) : 4747 - 4756
[46] Antimicrobial activity and chemical composition of white birch (Betula papyrifera Marshall) bark extracts
Blondeau, Dorian
St-Pierre, Annabelle
Bourdeau, Nathalie
Bley, Julien
Lajeunesse, Andre
Desgagne-Penix, Isabel
MICROBIOLOGYOPEN, 2020, 9 (01):
[47] Molecular composition and biological activity of commercial birch pollen allergen extracts
Focke, M.
Marth, K.
Valenta, R.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2009, 39 (05) : 429 - 436
[48] DIRECT METHYLATION OF SALTS OF ACIDS IN HYDROLYSIS MIXTURES OF NATURAL-PRODUCTS BY PHASE-TRANSFER CATALYSIS - AN INVESTIGATION OF THE SUBERIN FRACTION OF BIRCH BARK (BETULA-VERRUCOSA ERH )
BOLMGREN, I
NORIN, T
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1981, 35 (10): : 742 - 742
[49] CHARACTERIZATION OF THE ALDER (ALNUS-INCANA) POLLEN EXTRACT BY IEF AND 2-D PAGE IN COMPARISON TO BIRCH (BETULA-VERRUCOSA) AND HAZEL (CORYLUS-AVELLANA) - IDENTIFICATION OF ANTIGENS AND ALLERGENS BY ELECTROTRANSFER AND I-125 IMMUNOAUTORADIOGRAPHY
FLORVAAG, E
HOLEN, E
VIK, H
ELSAYED, S
ANNALS OF ALLERGY, 1985, 55 (02): : 381 - 381
[50] Can serum white birch (Betula verrucosa) pollen antigen (Bet v 1) immunoglobulin E measurement distinguish between sensitization and allergy?
Ciprandi, Giorgio
Comite, Paola
Ferrero, Francesca
Minale, Paola
Voltolini, Susanna
Bignardi, Donatella
Fontana, Vincenzo
Bruzzone, Marco
Troise, Costantino
Mussap, Michele
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY, 2015, 5 (12) : 1151 - 1155

← 1 2 3 4 5 →