FLUORESCENCE LIFETIME-BASED SENSING IN TISSUES - A COMPUTATIONAL STUDY

被引：77
|
作者：
HUTCHINSON, CL
LAKOWICZ, JR
SEVICKMURACA, EM
机构：
[1] PURDUE UNIV,SCH CHEM ENGN,W LAFAYETTE,IN 47907
[2] UNIV MARYLAND,DEPT BIOL CHEM,CTR FLUORESCENCE SPECT,BALTIMORE,MD 21201
来源：
BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 04期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
D O I：
10.1016/S0006-3495(95)80330-9
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
We have numerically solved the photon diffusion equation to predict the distribution of light ina tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption nd scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.
引用
收藏
页码：1574 / 1582
页数：9
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