DEVELOPMENT OF NA+-CURRENTS AND K+-CURRENTS IN THE COCHLEAR GANGLION OF THE CHICK-EMBRYO

被引:24
|
作者
VALVERDE, MA
SHEPPARD, DN
REPRESA, J
GIRALDEZ, F
机构
[1] UNIV VALLADOLID,FAC MED,DEPT BIOQUIM & BIOL MOLEC & FISIOL,E-47005 VALLADOLID,SPAIN
[2] UNIV VALLADOLID,FAC MED,DEPT CIENCIAS MORFOL,E-47005 VALLADOLID,SPAIN
关键词
D O I
10.1016/0306-4522(92)90301-H
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The development of Na+- and K+-currents in the primary afferent neurons of the cochlear ganglion was studied using the patch-clamp technique. Cells were dissociated between days 6 and 17 of development and membrane currents recorded within the following 24 h. Outward currents were the first to appear between days 6 and 7 of embryonic development and their magnitude increased throughout development from 200 pA on day 7 to 900 pA on days 14-16. Threshold for activation decreased by 20 mV between days 8 and 14. Outward currents were absent when Cs+ replaced k+ in the pipette and were partially blocked by external tetraethylammonium. Outward currents contained at least three components: (i) a non-inactivating outward current, similar to the delayed-rectifier, predominating in mature neurons; (ii) a slowly inactivating current (tau about 200 ms), most evident in early and intermediate stages (days 7-10); and (iii) a rapidly inactivating outward current (tau about 20 ms) similar to the A-current (I(A)) described in other neurons, which was distinctly expressed in mature neurons. Sodium currents were identified as fast transient inward currents, sensitive to tetrodotoxin and extracellular Na+-removal. They appeared later than K+-currents and increased in size from about 100 pA between days 9-11 to 600 pA by days 13-16. The development of membrane currents in cochlear ganglion neurons corresponded to defined stages of the innervation pattern of the chick cochlea [Whitehead and Morest (1985) Neuroscience 14, 255-2761. These currents could be functionally related to the establishment of synaptic connections between transducing cells and primary afferent neurons.
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页码:621 / 630
页数:10
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