HYDROPHOBICITY GRADIENT ON SILICA SURFACES - A STUDY USING TOTAL INTERNAL-REFLECTION FLUORESCENCE SPECTROSCOPY

被引：16
|
作者：
HLADY, V [1 ]
GOLANDER, C [1 ]
ANDRADE, JD [1 ]
机构：
[1] UNIV UTAH, CTR BIOPOLYMERS INTERFACES, SALT LAKE CITY, UT 84112 USA
来源：
COLLOIDS AND SURFACES | 1988年 / 33卷 / 1-2期
关键词：
ORGANIC COMPOUNDS - Adsorption - PROTEINS - Adsorption - SPECTROSCOPY - Applications - SURFACES - Spectroscopic Analysis;
D O I：
10.1016/0166-6622(88)80060-X
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
A silica surface with hydrophobicity gradient was characterized using total internal reflection spectroscopy and ANS, 1-anilinonaphthalene-8-sulfonate, as a surface probe. The probe responded to the hydrophobicity gradient of the surface. Strong enhancement of ANS fluorescence was found at the hydrophobic side of the gradient and no fluorescence enhancement at the hydrophilic side. Similar results were obtained with ANS binding to bovine serum albumin adsorbed on the gradient surface.
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页码：185 / 190
页数：6
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