DETECTION OF HUMAN HERPESVIRUS-6 IN PARAFFIN-EMBEDDED TISSUE OF CERVICAL-CANCER BY POLYMERASE CHAIN-REACTION

被引：16
|
作者：
WANG, H
CHEN, M
BERNEMAN, ZN
DELGADO, G
DIPAOLO, JA
机构：
[1] NCI, BIOL LAB, BETHESDA, MD 20892 USA
[2] NCI, TUMOR CELL BIOL LAB, BETHESDA, MD 20892 USA
[3] LOYOLA UNIV, STRITCH SCH MED, DEPT OBSTET & GYNECOL, MAYWOOD, IL 60153 USA
来源：
JOURNAL OF VIROLOGICAL METHODS | 1994年 / 47卷 / 03期
基金：
美国国家卫生研究院;
关键词：
POLYMERASE CHAIN REACTION; HHV-6; PARAFFIN-EMBEDDED TISSUE; CERVICAL CANCER;
D O I：
10.1016/0166-0934(94)90026-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The polymerase chain reaction (PCR) was used to detect human herpesvirus-6 (HHV-6) DNA sequences from paraffin-embedded tissue from cervical cancer patients. Two of eight cases were positive for HHV-6 using two sets of HHV-6 primers. Hybridization of PCR products with specific radioisotope-labeled oligonucleotide probes confirmed the results. Furthermore, HHV-6 typing was possible by adapting restriction endonuclease digestion of PCR product. This method is useful for retrospective studies in investigating the etiologic role of HHV-6 in the development of human diseases.
引用
收藏
页码：297 / 305
页数：9
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