5'-PHOSPHORYLATION OF OLIGONUCLEOTIDES WITH PHOSPHORUS-ACID IN AUTOMATED DNA-SYNTHESIS

被引：1
|
作者：
MARSTERS, JC
NG, P
JHURANI, P
VASSER, M
BISCHOFBERGER, N
机构：
[1] Genentech. Inc., Department of Molecular Biology, South San Francisco, CA 94080
来源：
NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 08期
关键词：
D O I：
10.1080/07328319008046064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coupling of phosphorous acid in automated DNA synthesis using H-phosphonate methodology leads to 5'-5' linked dimers and 5'-H-phosphonates. The yield is dependent on the phosphorous acid concentration, chain length of the oligomer, and pore size of the support. 5'-Phosphate oligomers are obtained from the H-phosphonate oligomers by silylation and oxidation.
引用
收藏
页码：1079 / 1086
页数：8
相关论文
共 50 条
  • [31] HISTONE PHOSPHORYLATION AND DNA-SYNTHESIS ARE LINKED IN SYNCHRONOUS CULTURES OF HTC CELLS
    BALHORN, R
    CHALKLEY, R
    BORDWELL, J
    SELLERS, L
    GRANNER, D
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 46 (03) : 1326 - +
  • [32] STRUCTURE-FUNCTION ANALYSIS OF MONONUCLEOTIDES AND SHORT OLIGONUCLEOTIDES IN THE PRIMING OF ENZYMATIC DNA-SYNTHESIS
    NEVINSKY, GA
    VENIAMINOVA, AG
    LEVINA, AS
    PODUST, VN
    LAVRIK, OI
    HOLLER, E
    BIOCHEMISTRY, 1990, 29 (05) : 1200 - 1207
  • [33] ELECTROCHEMICALLY INDUCED PROCESSES OF FORMATION OF PHOSPHORUS-ACID DERIVATIVES .4. SYNTHESIS OF TRIALKYL PHOSPHATES FROM WHITE PHOSPHORUS
    BUDNIKOVA, YG
    KARGIN, YM
    ZARIPOV, IM
    ROMAKHIN, AS
    IGNATEV, YA
    NIKITIN, EV
    TOMILOV, AP
    BULLETIN OF THE RUSSIAN ACADEMY OF SCIENCES-DIVISION OF CHEMICAL SCIENCE, 1992, 41 (09): : 1585 - 1588
  • [34] PROBABLE MECHANISM FOR APURINIC ACID INHIBITION OF DNA-SYNTHESIS
    FURLONG, NB
    THOMAS, FB
    MINTZ, CG
    FEBS LETTERS, 1974, 41 (02) : 256 - 258
  • [35] REGULATION OF DNA-SYNTHESIS BY AMINO-ACID LIMITATION
    BRUNNER, M
    CANCER RESEARCH, 1973, 33 (01) : 29 - 32
  • [36] STIMULATION OF EPIDERMAL DNA-SYNTHESIS BY VITAMIN A-ACID
    CHRISTOPHERS, E
    BRAUNFAL.O
    ARCHIV FUR KLINISCHE UND EXPERIMENTELLE DERMATOLOGIE, 1968, 232 (04): : 427 - +
  • [37] DNA-SYNTHESIS AFTER UNILATERAL AND 5/6 NEPHRECTOMY
    MISKELL, CA
    SIMPSON, DP
    CLINICAL RESEARCH, 1987, 35 (03): : A553 - A553
  • [38] EFFECTS OF 5-FLUOROURACIL ON PROSTATIC DNA-SYNTHESIS
    SLOAN, WR
    COFFEY, DS
    SCOTT, WW
    INVESTIGATIVE UROLOGY, 1975, 13 (02): : 90 - 94
  • [39] AURINTRICARBOXYLIC ACID (ATA) AND DNA-SYNTHESIS .1. INHIBITION OF DNA-SYNTHESIS BY ATA IN GO CELLS STIMULATED TO PROLIFERATE
    NOVI, AM
    VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY, 1977, 23 (02) : 137 - 154
  • [40] ELECTROCHEMICALLY INDUCED PROCESSES IN THE FORMATION OF PHOSPHORUS-ACID DERIVATIVES .1. SYNTHESIS OF TRIALKYL PHOSPHATES FROM WHITE PHOSPHORUS
    ROMAKHIN, AS
    BUDNIKOVA, YG
    ZARIPOV, IM
    KARGIN, YM
    NIKITIN, EV
    TOMILOV, AP
    IGNATEV, YA
    SMIRNOV, VV
    BULLETIN OF THE RUSSIAN ACADEMY OF SCIENCES-DIVISION OF CHEMICAL SCIENCE, 1992, 41 (06): : 1031 - 1035
12345