5'-PHOSPHORYLATION OF OLIGONUCLEOTIDES WITH PHOSPHORUS-ACID IN AUTOMATED DNA-SYNTHESIS

被引：1
|
作者：
MARSTERS, JC
NG, P
JHURANI, P
VASSER, M
BISCHOFBERGER, N
机构：
[1] Genentech. Inc., Department of Molecular Biology, South San Francisco, CA 94080
来源：
NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 08期
关键词：
D O I：
10.1080/07328319008046064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coupling of phosphorous acid in automated DNA synthesis using H-phosphonate methodology leads to 5'-5' linked dimers and 5'-H-phosphonates. The yield is dependent on the phosphorous acid concentration, chain length of the oligomer, and pore size of the support. 5'-Phosphate oligomers are obtained from the H-phosphonate oligomers by silylation and oxidation.
引用
收藏
页码：1079 / 1086
页数：8
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