The Mississippi Valley-type sphalerite mineralization in the Mascot-Jefferson City zinc district of East Tennessee occurs as open-space fillings in breccia bodies within the upper part of the Knox Group (Lower Ordovician) which is truncated by a regional unconformity. A lower age limit of mineralization is constrained by the formation of solution-collapse breccia bodies, which are believed to be related to the post-Knox unconformity. The breccias contain irregularly distributed ''sand'' bodies that represent cavities filled with well-laminated and size-graded, sphalerite-bearing, detrital, internal sediments. The texture, composition, and fluid inclusion characteristics of the sphalerite, are consistent with its local derivation from the wallrocks as detrital grains. The conformability between the laminations in the sediments and the bedding planes of the host carbonate rocks suggests that the sand bodies formed prior to the regional deformation event (Alleghenian orogeny). The stylolitization of carbonate and sphalerite clasts in the internal sediments as well as the deformation of the sphalerite are also consistent with a pre-Alleghenian age for the emplacement of the main-stage sphalerite mineralization in the Mascot-Jefferson City district and, by analogy, in other Lower Ordovician-hosted Mississippi Valley-type districts of the southern Appalachians.