Spectrophotometric Determination of UO22+ by a New Chemosensor Labetalol Hydrochloride

This work presents a new chemosensor Labetalol (LBT) hydrochloride 5-[1-hydroxy-2-(1-methyl-3-phenylpropylamino) ethyl] salicylamide hydrochloride (LBT) was applied for spectrophotometric determination of UO22+ in aqueous solution. The chemosensor LBT react with UO22+ forming a new complex UO22+/LBT at wavelength of 314 nm. The absorbance of the linear calibration curve increases by increasing UO22+ concentration at linear range of (1.0 - 16.1) x 10(-6) mol L-1 with a correlation coefficient (R-2 = 0.998). The experimental conditions affecting the absorbance development were studied and optimized. The complexes stoichiometry was measured at the absorbance spectra 314 nm and the results were found to be 1:1 (metal: ligand) ratio. The present chemosensor LBT was effectively validated with respect to linearity, precision, accuracy, detection and quantification limits. For more accurate analysis, the apparent molar absorptivity, Beer's law, Ringbom and Sandell's sensitivity have been studied. The results of analysis were statistically compared with those obtained by ICP-OES as a reference method show that, the Student's t- and F-values at 95% confidence level are less than the theoretical values, which confirmed that there is no significant difference between the chemosensor LBT and the reference method in the same studied conditions. The chemosensor LBT provide a simple, sensitive and inexpensive spectrophotometric determination without any complicated equipment. It was also successfully applied for the direct determination of UO22+ in different aqueous samples with satisfactory results.