VITELLOGENIN INDUCTION BY ESTRADIOL-17-BETA IN PRIMARY HEPATOCYTE CULTURE IN THE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS

被引：54

作者：

KWON, HC ^{[1
]}

HAYASHI, S ^{[1
]}

MUGIYA, Y ^{[1
]}

机构：

[1] KAGOSHIMA UNIV, FAC FISHERIES, KAGOSHIMA 890, JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 104卷 / 02期

关键词：

D O I：

10.1016/0305-0491(93)90383-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro induction of vitellogenin in response to estradiol-17beta was achieved in hepatocyte culture in male rainbow trout. 2. Estradiol-induced protein was identified as vitellogenin by immunoblot and immunoelectrophoresis, Its main subunit was estimated to be a molecular weight (MW) of 175,000 by SDS polyacrylamide gel electrophoresis. 3. An ELISA method showed that vitellogenin levels in the culture medium consistently increased throughout an experimental period of 5 days. 4. Triiodothyronine had no stimulative effect on the induction of vitellogenin.

引用

页码：381 / 386

页数：6

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