A FLUORESCENCE ASSAY DEMONSTRATING STIMULATION OF PHAGOCYTOSIS BY HEMOLYMPH MOLECULES OF GALLERIA-MELLONELLA

被引：57
|
作者：
ROHLOFF, LH
WIESNER, A
GOTZ, P
机构：
[1] Institut für Zoologie, Freie Universität Berlin, 14195 Berlin
来源：
JOURNAL OF INSECT PHYSIOLOGY | 1994年 / 40卷 / 12期
关键词：
INSECT IMMUNITY; INSECT HEMOCYTES; FLUORESCENCE; FITC-LABELED YEAST CELLS; PHAGOCYTOSIS ASSAY; GALLERIA MELLONELLA; STIMULATION OF PHAGOCYTOSIS; ULTRAFILTRATION; HEMOLYMPH MOLECULES;
D O I：
10.1016/0022-1910(94)90057-4
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
An in vitro microscopic fluorescence assay determining the phagocytic activity of isolated plasmatocytes of Galleria mellonella is described. It was developed to quantify insect cellular immune reactions The assay, a modification of a method originally established for vertebrate blood cells, is based on the quenching effect of trypan blue on fluorescein-isothiocyanate-labelled yeast cells. Only ingested yeast cells retain their fluorescence after quenching and can be easily distinguished from adhering ones. The technique is highly reproducible and easy to perform. Using this method a phagocytosis-stimulating effect caused by a haemolymph fraction > 100 kDa isolated from G. mellonella is demonstrated.
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页码：1045 / 1049
页数：5
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