ATP BINDING TO BOVINE SERUM-ALBUMIN

被引：22
|
作者：
BAUER, M [1 ]
BAUMANN, J [1 ]
TROMMER, WE [1 ]
机构：
[1] UNIV KAISERSLAUTERN,FACHBEREICH CHEM,POSTFACH 3049,W-6750 KAISERSLAUTERN,GERMANY
来源：
FEBS LETTERS | 1992年 / 313卷 / 03期
关键词：
BOVINE SERUM ALBUMIN; ATP; SPIN-LABELED-ATP; BINDING STUDIES; FATTY ACID; DOXYL-STEARATE;
D O I：
10.1016/0014-5793(92)81211-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific binding of ATP to bovine serum albumin (BSA) is demonstrated employing ATP derivatives spin-labeled at either N6 or C8 of adenine ring or at the ribose moiety. Based on a 1:1 stoichiometry binding constants are in the 50-100 muM range. Binding is largely competitive with ATP or stearic acid. A small fraction of the labeled nucleotides could not be liberated by these ligands. Binding of AMP is in the millimolar range, only.
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页码：288 / 290
页数：3
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