BIOTINYLATION OF OLIGONUCLEOTIDES AND THEIR USE AS POLYMERASE CHAIN-REACTION PRIMERS

被引：4
|
作者：
BENGTSTROM, M
HARJU, L
SYVANEN, AC
机构：
[1] Orion Corp, Orion Pharmaceutica, Biotechnology, 00380 Helsinki
来源：
NUCLEOSIDES & NUCLEOTIDES | 1991年 / 10卷 / 1-3期
关键词：
D O I：
10.1080/07328319108046510
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligonucleotides aminated at the 5'-end were biotinylated with a water soluble N-hydroxysuccinimide ester of biotin in large scale. The biotinylated oligonucleotides were used as polymerase chain reaction (PCR) primers.
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页码：507 / 509
页数：3
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