DETERMINATION OF PARTITION-COEFFICIENTS OF LABILE COMPOUNDS SUBJECT TO DISSOCIATION EXTRACTION - ANALYSIS OF PARTITIONING

被引：1
|
作者：
CRELIER, S [1 ]
MARGOT, A [1 ]
FLASCHEL, E [1 ]
RENKEN, A [1 ]
机构：
[1] ECOLE POLYTECH FED LAUSANNE, INST GENIE CHIM, CH-1015 LAUSANNE, SWITZERLAND
来源：
BIOCATALYSIS | 1993年 / 8卷 / 01期
关键词：
PARTITION COEFFICIENT; PH-EFFECT; DISSOCIATION EXTRACTION; PHENYLALANINE ESTER; AQUEOUS-ORGANIC 2-PHASE SYSTEM; TITRATION;
D O I：
10.3109/10242429309030956
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method for the determination of partition coefficients in aqueous-organic two-phase systems is described. It is applicable for characterizing the distribution of substances undergoing proton dissociation in the aqueous phase. The method is based on titration by means of the organic phase. A linear form of the respective model is given for parameter estimation by linear regression. This strategy is used for the determination of the partition coefficients of some N-unprotected n-alkyl esters of phenylalanine in various aqueous-organic biphasic systems. These esters are of interest for the optical resolution of phenylalanine with chymotrypsin.
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页码：59 / 71
页数：13
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