A NEW AFFINITY ADSORBENT FOR THE PURIFICATION OF PHOSPHOLIPASE-A1 AND PHOSPHOLIPASE-A2 FROM ANIMAL VENOMS

被引：3
|
作者：
VARGASVILLARREAL, J [1 ]
MARTINPOLO, JJ [1 ]
REYNAUD, E [1 ]
ALAGON, AC [1 ]
机构：
[1] UNIV NACL AUTONOMA MEXICO, CTR INVEST INGN GENET & BIOTECNOL, APDO POSTAL 510-3, CUERNAVACA 62270, MORELOS, MEXICO
来源：
TOXICON | 1991年 / 29卷 / 01期
关键词：
D O I：
10.1016/0041-0101(91)90044-R
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Dimethyl-DL-2,3-disteroyloxypropyl-2'-hydroxy-ethylammonium (Rosenthal's inhibitor) was coupled to carboxyhexyl-Sepharose 4B, through carbodiimide chemistry. Phopholipase A2 from Heloderma horridum horridum and Crotalus adamanteus bind to the immobilized ligand in the presence of Ca2+ and can be easily eluted under acidic conditions or in the presence of a cheating agent, respectivley. This affinity media proved to be effective also in the purification of a Ca2+-independent phospholipase A1 from vespid venom.
引用
收藏
页码：119 / 124
页数：6
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