Isolation and characterization of the Drosophila melanogaster gene encoding the vacuolar ATPase subunit A

被引:0
|
作者
Choi, KR [1 ]
Lee, KS [1 ]
Oh, Y [1 ]
Baek, K [1 ]
Yoon, J [1 ]
机构
[1] KYUNG HEE UNIV,DEPT & INST GENET ENGN,SUWON 449701,SOUTH KOREA
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中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Drosophila melanogaster gene (dvha) encoding a vacuolar ATPase subunit A has been isolated and characterized, The gene encodes a protein which is 607 amino acids long, The deduced amino acid sequence of the cloned gene is 86.5 and 82.1% identical to those of Manduca sexta and bovine vacuolar ATPase subunit A, respectively, Analysis of a genomic clone revealed that there are three coding exons, RNA blot analysis detected two transcripts; a 2.9 Mb transcript present in both adult heads and bodies; a 0.5 kb transcript present predominantly in adult heads.
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页码:544 / 548
页数:5
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