Isolation and characterization of the Drosophila melanogaster gene encoding the vacuolar ATPase subunit A

被引：0
|
作者：
Choi, KR [1 ]
Lee, KS [1 ]
Oh, Y [1 ]
Baek, K [1 ]
Yoon, J [1 ]
机构：
[1] KYUNG HEE UNIV,DEPT & INST GENET ENGN,SUWON 449701,SOUTH KOREA
来源：
MOLECULES AND CELLS | 1995年 / 5卷 / 06期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A Drosophila melanogaster gene (dvha) encoding a vacuolar ATPase subunit A has been isolated and characterized, The gene encodes a protein which is 607 amino acids long, The deduced amino acid sequence of the cloned gene is 86.5 and 82.1% identical to those of Manduca sexta and bovine vacuolar ATPase subunit A, respectively, Analysis of a genomic clone revealed that there are three coding exons, RNA blot analysis detected two transcripts; a 2.9 Mb transcript present in both adult heads and bodies; a 0.5 kb transcript present predominantly in adult heads.
引用
收藏
页码：544 / 548
页数：5
相关论文
共 50 条
  • [1] The Drosophila melanogaster gene vha14 encoding a 14-kDa F-subunit of the vacuolar ATPase
    Guo, YQ
    Kaiser, K
    Wieczorek, H
    Dow, JAT
    GENE, 1996, 172 (02) : 239 - 243
  • [2] Analysis and inactivation of vha55, the gene encoding the vacuolar ATPase B-subunit in Drosophila melanogaster reveals a larval lethal phenotype
    Davies, SA
    Goodwin, SF
    Kelly, DC
    Wang, ZS
    Sozen, MA
    Kaiser, K
    Dow, JAT
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (48) : 30677 - 30684
  • [3] Identification and characterization of the gene encoding a second proteolipid subunit of human vacuolar H+-ATPase (V-ATPase)
    Nishigori, H
    Yamada, S
    Takeda, J
    DIABETES, 1998, 47 : A411 - A411
  • [4] ISOLATION OF THE VMA-4 GENE ENCODING THE 26 KDA SUBUNIT OF THE NEUROSPORA-CRASSA VACUOLAR ATPASE
    BOWMAN, EJ
    STEINHARDT, A
    BOWMAN, BJ
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1237 (01): : 95 - 98
  • [5] ISOLATION AND CHARACTERIZATION OF A GENE ENCODING DNA TOPOISOMERASE-I IN DROSOPHILA-MELANOGASTER
    BROWN, SD
    HUANG, PC
    HSIEH, T
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 169 - 169
  • [6] ISOLATION AND CHARACTERIZATION OF A GENE ENCODING DNA TOPOISOMERASE-I IN DROSOPHILA-MELANOGASTER
    HSIEH, TS
    BROWN, SD
    HUANG, P
    FOSTEL, J
    NUCLEIC ACIDS RESEARCH, 1992, 20 (23) : 6177 - 6182
  • [7] Structure of the VPATPD gene encoding subunit D of the human vacuolar proton ATPase
    Agarwal, AK
    White, PC
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 279 (02) : 543 - 547
  • [8] Isolation and characterization of the Drosophila melanogaster cDNA encoding the sepiapterin reductase
    Seong, C
    Kim, YA
    Chung, HJ
    Park, D
    Yim, J
    Baek, K
    Park, YS
    Han, K
    Yoon, J
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1443 (1-2): : 239 - 244
  • [9] Isolation and characterization of the droPIK57 gene encoding a new regulatory subunit of phosphatidylinositol 3-kinase from Drosophila melanogaster
    Albert, S
    Twardzik, T
    Heisenberg, M
    Schneuwly, S
    GENE, 1997, 198 (1-2) : 181 - 189
  • [10] Isolation and characterization of a new gene encoding a member of the HIRA family of proteins from Drosophila melanogaster
    Kirov, N
    Shtilbans, A
    Rushlow, C
    GENE, 1998, 212 (02) : 323 - 332
12345