INTERLEUKIN-6 EXPRESSION BY FIBROBLASTS GROWN IN 3-DIMENSIONAL GEL CULTURES

被引：18
|
作者：
ECKES, B
HUNZELMANN, N
ZIEGLERHEITBROCK, HWL
URBANSKI, A
LUGER, T
KRIEG, T
MAUCH, C
机构：
[1] UNIV COLOGNE,DEPT DERMATOL,JOSEPH STELZMANN STR 9,W-5000 COLOGNE 41,GERMANY
[2] UNIV MUNICH,INST IMMUNOL,W-8000 MUNICH 2,GERMANY
[3] UNIV MUNSTER,DEPT DERMATOL,LBI DVS,CELLBIOL LAB,W-4400 MUNSTER,GERMANY
来源：
FEBS LETTERS | 1992年 / 298卷 / 2-3期
关键词：
INTERLEUKIN-6; EXTRACELLULAR MATRIX; 3-DIMENSIONAL COLLAGEN GEL; FIBROBLAST;
D O I：
10.1016/0014-5793(92)80064-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigated the expression and biological activity of interleukin-6 (IL-6) by human fibroblasts cultured as monolayers and within three-dimensional type I collagen lattices. In the course of contracting the gel to a dense tissue-like structure, the cells upregulated their levels of IL-6 mRNA as well as IL-6 biological activity. While there was little mRNA and protein activity (6,500 U/ml) in monolayer cultures, fibroblasts in the 3D system showed a 13-fold increase in IL-6 mRNA on day 3. IL-6 protein was increased 6-fold (38,000 U/ml) on day 4. Stimulation of fibroblast cultures with IL-1-alpha resulted in enhanced IL-6 production in both systems, but the fibroblasts embedded into the 3D network continued to exhibit higher levels.
引用
收藏
页码：229 / 232
页数：4
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