Simpler and Faster Spectrophotometric Determination of Diclofenac Sodium in Tablets, Serum and Urine Samples

This work describes a simple, sensitive, rapid and economical analytical procedure for direct spectrophotometric evaluation of diclofenac sodium (DS) using aqueous medium without using a chemical reagent. Parameters like time, temperature, acidic and basic conditions and interference by analgesic drugs were studied for a 5 mu g ml(-1) solution of DS at 276 nm. Under optimized parameters, a linear working range of 0.1-30 mu g ml(-1) with regression coefficient of 0.9998 and lower detection limit of 0.01 mu g ml(-1) was obtained. The method was applied for DS contents in tablets, serum and urine samples.