Determination of aglucone isoflavones in non-transgenic and transgenic soybeans (GLYCINE MAX L.) by HPLC-DAD

被引：0
|
作者：
Ribani, Marcelo [1 ,2 ]
Ribani, Rosesemary H. [3 ]
do Valle, Tatiane [2 ]
Guilherme, Melissa A. W. [2 ]
Collins, Carol H. [1 ]
Bottoli, Carla B. G. [1 ]
机构：
[1] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13084970 Campinas, SP, Brazil
[2] TECPAR Technol Inst Parana, BR-80035060 Curitiba, PR, Brazil
[3] Univ Fed Parana, Dept Chem Engn, BR-81531970 Curitiba, PR, Brazil
来源：
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 1卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
soybeans; Glycine max L; aglucone isoflavones; transgenic; HPLC-DAD;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Soybeans, an important global crop, is an excellent source of oil and proteins and also contain many other compounds, including isoflavones, which have been the subject of several studies demonstrating their potential in the treatment of menopause and osteoporosis. This paper describes a method for quantification of the aglucone isoflavones found in soybeans using high performance liquid chromatography with diode array detection after extraction of the total isoflavones followed by acid hydrolysis. The quantities of the total aglucone isoflavones (daidzein, glycitein and genistein) in non-transgenic (BRS133) and transgenic (BRS245RR) soybeans, planted in the same region (north of Parana State, Brazil) and in the same period of the year, in 100 g of soybeans, were 284 +/- 10 mg and 228 +/- 13 mg for the non-transgenic and transgenic soybean varieties, respectively.
引用
收藏
页码：92 / 97
页数：6
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