PURIFICATION AND N-TERMINAL AMINO-ACID-SEQUENCE OF THE TARTRATE-RESISTANT ACID-PHOSPHATASE FROM HUMAN OSTEOCLASTOMA - EVIDENCE FOR A SINGLE STRUCTURE

被引：23
|
作者：
STEPAN, JJ
LAU, KHW
MOHAN, S
SINGER, FR
BAYLINK, DJ
机构：
[1] UNIV SO CALIF,LOS ANGELES,CA 90089
[2] ORTHOPED HOSP,LOS ANGELES,CA
[3] LOMA LINDA UNIV,DEPT MED,LOMA LINDA,CA 92350
[4] LOMA LINDA UNIV,DEPT PHYSIOL & BIOCHEM,LOMA LINDA,CA 92350
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)92391-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tartrate-resistant acid phosphatase type-5 was purified to apparent homogeneity from human osteoclastomas by sequential chromatography on CM-Sepharose, Phenyl-Sepharose, concanavalin A-Sepharose, FPLC Superose-12, and FPLC Mono-S. The purification over the original tissue extract was 1167-fold, with a yield of 16%. An identity in the N-terminal amino acid sequence and Mr was found between this enzyme and two type-5 tartrate-resistant acid phosphatases isolated from hairy cell leukemia spleen. However, they appeared to be different as assessed by amino acid composition. In contrast to a previous report, no evidence was found for two subunits of the tartrate-resistant acid phosphatase. © 1990.
引用
收藏
页码：792 / 800
页数：9
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