PURIFICATION AND FLUORESCENT PROPERTIES OF FLAVIN-CONTAINING LIVER POLYAMINE OXIDASE

被引：0
|
作者：
GASPARYAN, VK
NALBANDYAN, RM
机构：
来源：
BIOCHEMISTRY-MOSCOW | 1990年 / 55卷 / 09期
关键词：
POLYAMINE OXIDASE; FLAVIN; DENATURATION; QUENCHING;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A flavin-containing polyamine oxidase was obtained in an electrophoretically homogeneous state from bovine liver cytosol. The molecular weight, subunit composition, and flavin content were determined; the covalent nature of the bond of flavin to the protein portion was established. The optical and fluorescent properties of the enzyme in the native and denatured states were studied. An influence of denaturation and quenching agents on the fluorescent properties of the enzyme was demonstrated.
引用
收藏
页码：1223 / 1227
页数：5
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