USE OF ANTIOXIDANTS DURING PREPARATION OF ALPHA-TOCOPHEROL ENRICHED LIPOSOMES

被引:0
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作者
PEKINER, B
PENNOCK, JF
机构
[1] Department of Biochemistry, Faculty of Life Sciences, University of Liverpool
关键词
ASCORBIC ACID; BUTYLATED HYDROXYTOLUENE; ALPHA-TOCOPHEROL;
D O I
10.1016/0271-5317(95)00045-K
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Ultrasonic radiation caused a breakdown in alpha-tocopherol in the liposomal membranes. Some of the radiolabelled 5-(CH3)-C-14-alpha-tocopherol had been broken to alpha-tocopherylquinone (18.7 +/- 1.57 %) and to a less polar compound of alpha-tocopherol (13.1 +/- 1.29 %). When alpha-tocopherol liposomes were incubated overnight the remaining alpha-tocopherol was 9.32 +/- 1.35 %. Use of butylated hydroxytoluene in liposomes or in the incubation medium (outside liposomes) improved recovery of alpha-tocopherol to some extent. Butylated hydroxytoluene in the liposomal fraction protected 26.8 +/- 1.78 % of alpha-tocopherol, while in the incubation medium (outside the liposome) it protected 37.9 +/- 1.18 %. There was a shift from alpha-tocopherylquinone formation to a less polar compound of alpha-tocopherol formation when butylated hydroxytoluene was present both in liposome and in the incubation medium; the protection was increased to 53.2 +/- 1.61 %. However, ascorbate added to the incubation medium protected 86.4 +/- 1.45 % of labeled alpha-tocopherol. Our findings suggest that ascorbate is a better antioxidant than butylated hydroxytoluene during the preparation of alpha-tocopherol liposomes.
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页码:795 / 802
页数:8
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