PURIFICATION AND CHARACTERIZATION OF 2 DISTINCT LIPASES FROM CANDIDA-CYLINDRACEA

被引：207
|
作者：
RUA, ML [1 ]
DIAZMAURINO, T [1 ]
FERNANDEZ, VM [1 ]
OTERO, C [1 ]
BALLESTEROS, A [1 ]
机构：
[1] CSIC,INST QUIM FIS ROCASOLANO,MADRID 6,SPAIN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1156卷 / 02期
关键词：
LIPASE; ISOENZYME; ENZYME PURIFICATION; ENZYME CHARACTERIZATION; CANDIDA-CYLINDRACEA; CANDIDA-RUGOSA;
D O I：
10.1016/0304-4165(93)90134-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified and characterized two isoenzymes from a commercial lipase preparation of Candida cylindracea. The purification procedure includes ethanol precipitation and DEAE Sephacel and Sephacryl HR 100 chromatographies. Lipase A and lipase B were purified 11-fold with a 5% and 21% recovery in activity, respectively. The enzymes have similar amino acid content, N-terminal sequence and molecular weight, but differ on neutral sugar content, hydrophobicity, presence of isoforms and stability to pH and temperature. They also show some differences in the substrate specificity.
引用
收藏
页码：181 / 189
页数：9
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