The Caenorhabditis elegans rop-1 gene encodes the homologue of the human 60-kDa Ro autoantigen

被引：14
|
作者：
Labbe, JC [1 ]
Jannatipour, M [1 ]
Rokeach, LA [1 ]
机构：
[1] UNIV MONTREAL,DEPT BIOCHIM,MONTREAL,PQ H3C 3J7,CANADA
来源：
GENE | 1995年 / 167卷 / 1-2期
关键词：
epitopes; nematode; ribonucleoprotein; RNA-binding; SS-A; systemic lupus erythematosus;
D O I：
10.1016/0378-1119(95)00695-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
As a first step toward establishing a genetic system for the elucidation of the cellular role(s) of the Ro ribonucleoproteins (RoRNP), we have cloned the gene encoding the homologue of the human 60-kDa Ro protein (Ro60) in Caenorhabditis elegans (Ce). This Ce gene is present as a single copy and contains a 643-codon open reading frame interrupted by three introns. The encoded protein, Rop1p, shares 40% identity and 63% overall similarity with both the human and amphibian Ro60. Recombinant protein has been produced in Escherichia coli and used to elicit anti-Rop1p antibodies. Immunological analysis indicated that the Ro60 epitopes have been poorly conserved. Gene-fusion expression studies in transgenic nematodes will provide a new avenue of research to shed light on the function of these particles.
引用
收藏
页码：227 / 231
页数：5
相关论文
共 50 条
  • [21] The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing
    Ma, Long
    Gao, Xiaoyang
    Luo, Jintao
    Huang, Liange
    Teng, Yanling
    Horvitz, H. Robert
    PLOS GENETICS, 2012, 8 (07):
  • [22] The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers
    Chen, LC
    Manjeshwar, S
    Lu, Y
    Moore, D
    Ljung, BM
    Kuo, WL
    Dairkee, SH
    Wernick, M
    Collins, C
    Smith, HS
    CANCER RESEARCH, 1998, 58 (16) : 3677 - 3683
  • [23] THE CAENORHABDITIS-ELEGANS UNC-60 GENE ENCODES PROTEINS HOMOLOGOUS TO A FAMILY OF ACTIN-BINDING PROTEINS
    MCKIM, KS
    MATHESON, C
    MARRA, MA
    WAKARCHUK, MF
    BAILLIE, DL
    MOLECULAR AND GENERAL GENETICS, 1994, 242 (03): : 346 - 357
  • [24] The role of the presenilin-1 homologue gene sel-12 of Caenorhabditis elegans in apoptotic activities
    Kitagawa, A
    Shimohama, S
    Oeda, T
    Uemura, K
    Kohno, R
    Kuzuya, A
    Shibasaki, H
    Ishii, N
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (14) : 12130 - 12134
  • [25] The yeast SME1 gene encodes the homologue of the human E core protein
    Bordonne, R
    Tarassov, I
    GENE, 1996, 176 (1-2) : 111 - 117
  • [26] A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease
    Parker, AE
    Van de Weyer, I
    Laus, MC
    Oostveen, I
    Yon, J
    Verhasselt, P
    Luyten, WHML
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (29) : 18332 - 18339
  • [27] Cloning and developmental expression analysis of ltd-1, the Caenorhabditis elegans homologue of the mouse kyphoscoliosis (ky) gene
    Vargas, JD
    Culetto, E
    Ponting, CP
    Miguel-Aliaga, I
    Davies, KE
    Sattelle, DB
    MECHANISMS OF DEVELOPMENT, 2002, 117 (1-2) : 289 - 292
  • [28] Zonula adherens formation in Caenorhabditis elegans requires dlg-1, the homologue of the Drosophila gene discs large
    Bossinger, O
    Klebes, A
    Segbert, C
    Theres, C
    Knust, E
    DEVELOPMENTAL BIOLOGY, 2001, 230 (01) : 29 - 42
  • [29] The Caenorhabditis elegans hif-1 gene encodes a bHLH-PAS protein that is required for adaptation to hypoxia
    Jiang, HQ
    Guo, R
    Powell-Coffman, JA
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (14) : 7916 - 7921
  • [30] The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease
    Masson, JY
    Tremblay, S
    Ramotar, D
    GENE, 1996, 179 (02) : 291 - 293
12345