IDENTIFICATION OF 17-18 KDA ZONA-PELLUCIDA BINDING-PROTEINS FROM BOAR SPERMATOZOA

被引:23
|
作者
MOOS, J
PEKNICOVA, J
SURNEVANAKOVA, TN
PAVLIK, M
机构
[1] BULGARIAN ACAD SCI, INST BIOL & IMMUNOL REPROD & DEV ORGANISMS, BU-1113 SOFIA, BULGARIA
[2] CZECHOSLOVAK ACAD SCI, INST ORGAN CHEM & BIOCHEM, CS-16637 PRAGUE 6, CZECHOSLOVAKIA
关键词
Acrosin; Amino acid sequence; Boar sperm cell; Fucose; Zona pellucida binding protein;
D O I
10.1016/0014-5793(90)80258-K
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Zona pellucida (ZP) binding proteins from boar spermatozoa were compared with antigens recognized by ACR.2 and ACR.3 monoclonal antibodies. The ZP binding proteins of 55, 53,45 and 38 kDa are identical with various forms of boar acrosin immunologically detected by ACR.2 antibody. The ZP binding proteins of 17 and 18 kDa are recognized by ACR.3 antibody. The N-tenninal amino acid sequence of the 17 kDa protein revealed that it is not derived from an acrosin molecule. © 1990.
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页码:243 / 245
页数:3
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