A FATTY-ACID-BINDING PROTEIN FROM WHEAT KERNELS

被引：11
|
作者：
CASTAGNARO, A [1 ]
GARCIAOLMEDO, F [1 ]
机构：
[1] UNIV POLITECN MADRID,ETS INGENIEROS AGRONOMOS,BIOQUIM & BIOL MOLEC LAB,E-28040 MADRID,SPAIN
来源：
FEBS LETTERS | 1994年 / 349卷 / 01期
关键词：
CEREAL; FATTY ACID BINDING PROTEIN; PLANT; WHEAT;
D O I：
10.1016/0014-5793(94)00660-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A protein of about 7 kDa (W-FABP) has been isolated from mature wheat kernels by H2O extraction and gel filtration of the extract, followed by two steps of high-performance liquid chromatography. The N-terminal amino acid sequence has been determined up to the 28th residue and found to be identical (except for positions 4 and 5) to that deduced from a barley cDNA (EMBL X15257), which had been improperly classified as a non-specific lipid transfer protein (LTP2). Similarly with LTPs, W-FABP does bind fatty acids, but in contrast, it is not significantly homologous to LTPs, it is not recognized by LTP antibodies, it has a more acidic isoelectric point (pH < 6.8 vs. pH > 9.6), and it does not show antibiotic properties.
引用
收藏
页码：117 / 119
页数：3
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