Aim: To determination of mean serum calcium and mean lipid profile in patients with gall stone Total 380 patients were included in this cross sectional study after scrutinized by inclusion criteria and this study was conducted at Department of Surgery Nishter Hospital, Multan from September 2015 to March 2016. Male or female patients with gall stone disease having age from 20 to 70 years were included in this study. Results: Mean age of the patients was 43.18 +/- 13.970 years, mean serum calcium was 11.55 +/- 1.18mg/dl, mean serum HDL was 40.21 +/- 3.1mg/dl, mean serum LDL was 137.98 +/- 9.79mg/dl, mean serum TC was 204.27 +/- 24.12mg/dl, mean serum TG was 198.94 +/- 72.1mg/dl, mean weight was 54.30 +/- 13.07 kg and mean height was 63.57 +/- 4.14 inch. Conclusion: Results of this study revealed that female are more victim of gallstone disease as compare to male. It is also concluded that increased serum calcium and serum lipid have a role in the pathogenesis of different types of human gallstones in this area.