ISOLATION OF CD3(-), CD4(-), CD8(-), IL-2R(+) THYMOCYTE PRECURSORS BY PANNING

被引：5
|
作者：
SMALL, M
MAJUMDAR, AS
LIEBERMAN, M
WEISSMAN, I
机构：
[1] STANFORD UNIV, SCH MED, DEPT RADIAT ONCOL, STANFORD, CA USA
[2] STANFORD UNIV, SCH MED, DEPT PATHOL, STANFORD, CA USA
[3] STANFORD UNIV, SCH MED, DEPT DEV BIOL, STANFORD, CA USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 167卷 / 1-2期
关键词：
PANNING; PRECURSOR THYMOCYTE; LYMPHOCYTE;
D O I：
10.1016/0022-1759(94)90079-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a panning method for isolation of thymocytes that are CD3(-), CD4(-), CD8(-) and IL-2R(+). These cells have been isolated by positive selection on dishes coated with 7D4 antibody followed by treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.
引用
收藏
页码：103 / 107
页数：5
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