INVITRO CONFOCAL IMAGING OF THE RABBIT CORNEA

被引：29
|
作者：
MASTERS, BR [1 ]
PADDOCK, S [1 ]
机构：
[1] UNIV WISCONSIN, INTEGRATED MICROSCOPY RESOURCE BIOMED RES, MADISON, WI 53706 USA
来源：
JOURNAL OF MICROSCOPY | 1990年 / 158卷
关键词：
actin; basal lamina; Confocal microscopy; cornea; Descemet's membrane; nerve plexus; stromal keratocytes;
D O I：
10.1111/j.1365-2818.1990.tb03000.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We were able to observe in vitro the fine structure of the rabbit cornea using a laser scanning confocal microscope, especially in the regions between Descemet's membrane and the epithelial basal lamina. We observed submicrometre filaments throughout the stroma with high concentrations adjacent to Descemet's membrane, and found extensive interconnecting processes between stromal keratocytes. There are numerous regions containing nerve plexuses in the stroma. We found a deeply convoluted basal lamina adjacent to the epithelium, and observed regions containing junctions between endothelial cells in fluorescent images of rabbit corneas stained with the actin‐specific compound fluorescein phalloidin. 1990 Blackwell Science Ltd
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页码：267 / 274
页数：8
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