SKELETAL KERATAN SULFATE CHAIN MOLECULAR-WEIGHT CALIBRATION BY HIGH-PERFORMANCE GEL-PERMEATION CHROMATOGRAPHY

被引：16
|
作者：
DICKENSON, JM
MORRIS, HG
NIEDUSZYNSKI, IA
HUCKERBY, TN
机构：
[1] UNIV LANCASTER,INST ENVIRONM & BIOL SCI,DIV BIOL SCI,LANCASTER LA1 4YQ,ENGLAND
[2] UNIV LANCASTER,DEPT CHEM,LANCASTER LA1 4YA,ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 190卷 / 02期
关键词：
D O I：
10.1016/0003-2697(90)90192-C
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gelpermeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 m NaCl and at 30°C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship log10Mr = 4.588 - 2.128Kav which is applicable in the range Kav = 0.15-0.65. © 1990.
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页码：271 / 275
页数：5
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