VOLTAMMETRIC BEHAVIOR OF DIHYDRONICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE AT ENZYME-MODIFIED ELECTRODES

被引：0
|
作者：
CHI, H
WALSH, S
SMYTH, MR
SVANCARA, I
VYTRAS, K
机构：
[1] DUBLIN CITY UNIV, SCH CHEM SCI, DUBLIN 9, IRELAND
[2] UNIV CHEM TECHNOL, DEPT ANALYT CHEM, CS-53210 PARDUBICE, CZECHOSLOVAKIA
来源：
ELECTROANALYSIS | 1992年 / 4卷 / 01期
关键词：
NICOTINAMIDE COENZYMES; 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NADPH; MODIFIED ELECTRODES;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The oxidation of nicotinamide-adenine dinucleotide phosphate (reduced form, NADPH) at glassy carbon electrodes [modified with different enzymes has been studied. It was found that NADPH yielded a well-defined anodic peak about +0.70 V (vs. Ag/AgCl) at glassy carbon electrodes coated with three different enzymes, glutathione reductase, glutathione peroxidase, and glucose oxidase, in 1.4 M potassium phosphate supporting electrolyte. it was confirmed that the enzymes immobilized at the working electrode surface reduced the overpotential for the oxidation of NADPH. This method provides a way to study the mechanism of some enzymatic reactions.
引用
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页码：107 / 110
页数：4
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