THIDIAZURON-INDUCED HIGH-FREQUENCY OF SHOOT INDUCTION AND PLANT-REGENERATION IN PROTOPLAST-DERIVED PEA CALLUS

被引：0
|
作者：
BOHMER, P [1 ]
MEYER, B [1 ]
JACOBSEN, HJ [1 ]
机构：
[1] UNIV HANNOVER, LEHRGEBIET MOLEK GENET, D-30419 HANNOVER, GERMANY
来源：
PLANT CELL REPORTS | 1995年 / 15卷 / 1-2期
关键词：
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1 cm were transferred to shoot induction media, containing different concentrations of thidiazuron. Shoot formation was after 16 weeks up to 12% efficiency. Thidiazuron (10 mu M) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
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页码：26 / 29
页数：4
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