THIDIAZURON-INDUCED HIGH-FREQUENCY OF SHOOT INDUCTION AND PLANT-REGENERATION IN PROTOPLAST-DERIVED PEA CALLUS

被引：0
|
作者：
BOHMER, P [1 ]
MEYER, B [1 ]
JACOBSEN, HJ [1 ]
机构：
[1] UNIV HANNOVER, LEHRGEBIET MOLEK GENET, D-30419 HANNOVER, GERMANY
来源：
PLANT CELL REPORTS | 1995年 / 15卷 / 1-2期
关键词：
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1 cm were transferred to shoot induction media, containing different concentrations of thidiazuron. Shoot formation was after 16 weeks up to 12% efficiency. Thidiazuron (10 mu M) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
引用
收藏
页码：26 / 29
页数：4
相关论文
共 50 条
  • [1] Shoot differentiation and plant regeneration from thidiazuron-induced callus of Salvia officinalis
    Tawfik, A. A.
    Mohamed, M. F.
    [J]. PROCEEDINGS OF THE 1ST INTERNATIONAL SYMPOSIUM ON THE LABIATAE: ADVANCES IN PRODUCTION, BIOTECHNOLOGY AND UTILISATION, 2006, (723): : 309 - +
  • [2] IMPROVED PROTOPLAST CULTURE AND PLANT-REGENERATION FROM PROTOPLAST-DERIVED CALLUS IN ARABIDOPSIS-THALIANA
    XUAN, LT
    MENCZEL, L
    [J]. ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1980, 96 (01): : 77 - 80
  • [3] Plant regeneration from protoplast-derived callus of sugarcane
    Matsuoka, M
    Sugimoto, A
    [J]. BREEDING SCIENCE, 1997, 47 (03) : 301 - 305
  • [4] PLANT-REGENERATION FROM PROTOPLAST-DERIVED CALLUS OF RICE (ORYZA-SATIVA-L)
    YAMADA, Y
    YANG, ZQ
    TANG, DT
    [J]. PLANT CELL REPORTS, 1986, 5 (02) : 85 - 88
  • [5] Thidiazuron-induced high-frequency axillary and adventitious shoot regeneration in Vigna radiata (L.) Wilczek
    S. Amutha
    M. Muruganantham
    A. Ganapathi
    [J]. In Vitro Cellular & Developmental Biology - Plant, 2006, 42
  • [6] Thidiazuron-induced high-frequency axillary and adventitious shoot regeneration in Vigna radiata (L.) Wilczek
    Amutha, S
    Muruganantham, M
    Ganapathi, A
    [J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2006, 42 (01) : 26 - 30
  • [7] Thidiazuron-induced high-frequency shoot proliferation in Cineraria maritima Linn.
    Banerjee, S
    Tripathi, J
    Verma, PC
    Dwivedi, PD
    Khanuja, SPS
    Bagchi, GD
    [J]. CURRENT SCIENCE, 2004, 87 (09): : 1287 - 1290
  • [8] CALLUS INDUCTION AND HIGH-FREQUENCY PLANT-REGENERATION IN ITALIAN MILLET (SETARIA-ITALICA)
    RAO, AM
    KISHOR, PBK
    REDDY, LA
    VAIDYANATH, K
    [J]. PLANT CELL REPORTS, 1988, 7 (07) : 557 - 559
  • [9] Thidiazuron-induced high-frequency shoot regeneration from root region of Robinia pseudoacacia L. seedlings
    Hosseini-Nasr, M
    Rashid, A
    [J]. BIOLOGIA PLANTARUM, 2003, 47 (04) : 593 - 596
  • [10] Thidiazuron-induced high-frequency plant regeneration from leaf explants of Paulownia tomentosa mature trees
    Corredoira, E.
    Ballester, A.
    Vieitez, A. M.
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 95 (02) : 197 - 208
12345