NEDOCROMIL SODIUM SELECTIVELY INHIBITS IGE AND IGG4 PRODUCTION IN HUMAN B-CELLS STIMULATED WITH IL-4

被引:0
|
作者
KIMATA, H
MIKAWA, H
机构
来源
JOURNAL OF IMMUNOLOGY | 1993年 / 151卷 / 12期
关键词
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We studied the effect of nedocromil sodium (NES) on human IgE and IgG subclass production. NES inhibited IgE and IgG4 production, without affecting IgM, IgA, IgG1, IgG2, or IgG3 production, by IL-4-stimulated mononuclear cells (MNC) obtained from non-atopic donors. Inhibition of IgE and IgG4 production by NES required the compound to be added on day 0 and to be present throughout the entire period of culture. Inhibition of IgE and IgG4 production was not mediated by known inhibiters, including IFN-alpha, IFN-gamma, transforming growth factor-beta, PGE2, or platelet-activating factor. Inhibition by NES required both T cells and monocytes because NES inhibited IgE production by B cells stimulated with IL-4 and anti-CD40 mAb in the presence, but not the absence, of T cells and monocytes. NES also inhibited IgE and IgG4 production in cultures stimulated with anti-CD3-treated T cells. However, in this case other isotypes were also inhibited by NES. In contrast to non-atopic donors' MNC, NES failed to inhibit spontaneous IgE and IgG4 production by MNC from atopic patients. However, NES was effective in inhibiting IL-4-induced IgE and IgG4 production in atopic patients' MNC without affecting IgM, IgA, IgG1, IgG2, or IgG3 production. NES inhibited IgE and IgG4 production by sIgE- and sIgG4- B cells, but not by sIgE+ and sIgG4+ B cells, respectively, stimulated with IL-4 or IL-4 plus anti-CD40 mAb in the presence of T cells and monocytes. These results suggest that NES may inhibit IL-4-induced switching of IgE and IgG4 production by sIgE- B cells and sIgG4- B cells, respectively, by affecting the interaction of B cells, T cells, and monocytes.
引用
收藏
页码:6723 / 6732
页数:10
相关论文
共 50 条
  • [1] DISODIUM-CROMOGLYCATE (DSCG) SELECTIVELY INHIBITS IGE PRODUCTION AND ENHANCES IGG4 PRODUCTION BY HUMAN B-CELLS INVITRO
    KIMATA, H
    YOSHIDA, A
    ISHIOKA, C
    MIKAWA, H
    [J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1991, 84 (03): : 395 - 399
  • [2] SUPPRESSION BY IL-2 OF IGE PRODUCTION BY B-CELLS STIMULATED BY IL-4
    MIYAJIMA, H
    HIRANO, T
    HIROSE, S
    KARASUYAMA, H
    OKUMURA, K
    OVARY, Z
    [J]. JOURNAL OF IMMUNOLOGY, 1991, 146 (02): : 457 - 462
  • [3] INTERLEUKIN-4 INDUCES SYNTHESIS OF IGE AND IGG4 IN HUMAN B-CELLS
    LUNDGREN, M
    PERSSON, U
    LARSSON, P
    MAGNUSSON, C
    SMITH, CIE
    HAMMARSTROM, L
    SEVERINSON, E
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (07) : 1311 - 1315
  • [4] INVOLVEMENT OF INTERLEUKIN (IL)-13, BUT NOT IL-4, IN SPONTANEOUS IGE AND IGG4 PRODUCTION IN NEPHROTIC SYNDROME
    KIMATA, H
    FUJIMOTO, M
    FURUSHO, K
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1995, 25 (06) : 1497 - 1501
  • [5] HYDROCORTISONE AND IL-4 INDUCE IGE ISOTYPE SWITCHING IN HUMAN B-CELLS
    JABARA, HH
    AHERN, DJ
    VERCELLI, D
    GEHA, RS
    [J]. JOURNAL OF IMMUNOLOGY, 1991, 147 (05): : 1557 - 1560
  • [6] THE EFFECTS OF IL-4 ON HUMAN B-CELLS
    DEFRANCE, T
    AUBRY, JP
    VANBERVLIET, B
    BONNEFOY, JY
    YOKOTA, T
    ARAI, K
    DEVRIES, JE
    BANCHEREAU, J
    [J]. IMMUNOBIOLOGY, 1986, 173 (2-5) : 149 - 149
  • [7] INTERLEUKIN-8 (IL-8) SELECTIVELY INHIBITS IMMUNOGLOBULIN-E PRODUCTION INDUCED BY IL-4 IN HUMAN B-CELLS
    KIMATA, H
    YOSHIDA, A
    ISHIOKA, C
    LINDLEY, I
    MIKAWA, H
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (04): : 1227 - 1231
  • [8] The effect of inhibitors of CD23 processing on the production of IgE and IgG from IL-4 stimulated human B cells and PBMC
    Mayer, RJ
    Al-Mahdi, N
    Bolognese, BJ
    Katchur, SR
    Eichman, C
    Marshall, LA
    Faller, A
    [J]. FASEB JOURNAL, 2000, 14 (06): : A1067 - A1067
  • [9] IL-4 INDUCES CO-EXPRESSION OF INTRINSIC MEMBRANE IGG1 AND IGE BY MURINE B-CELLS STIMULATED WITH LIPOPOLYSACCHARIDE
    SNAPPER, CM
    FINKELMAN, FD
    STEFANY, D
    CONRAD, DH
    PAUL, WE
    [J]. JOURNAL OF IMMUNOLOGY, 1988, 141 (02): : 489 - 498
  • [10] RANTES and macrophage inflammatory protein 1 alpha selectively enhance immunoglobulin (IgE) and IgG4 production by human B cells
    Kimata, H
    Yoshida, A
    Ishioka, C
    Fujimoto, M
    Lindley, I
    Furusho, K
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (05): : 2397 - 2402