COMPARISON OF METABOLISM OF HYBRIDOMA CELLS CULTURED IN MEDIA SUPPLEMENTED WITH WHEY OR FETAL CALF SERUM

被引：8

作者：

LEGRAND, C ^{[1
]}

CAPIAUMONT, J ^{[1
]}

BELLEVILLE, F ^{[1
]}

NABET, P ^{[1
]}

机构：

[1] IBN,NANCY,FRANCE

来源：

BIOTECHNOLOGY PROGRESS | 1993年 / 9卷 / 06期

关键词：

D O I：

10.1021/bp00024a002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of industrial whey on cell physiology were studied in a repeated fed-harvest mode using free-suspended murine hybridoma cells. After several days of culture in medium containing 9% whey and 1% fetal calf serum (FCS), cell growth and viability, carbohydrate, amino acid, energy metabolism, and antibody production rates were investigated. Differences were found between cells cultured in whey medium and those cultured in conventional FCS medium. The cell growth obtained in medium supplemented with fresh whey was similar to that obtained in FCS medium. The viability showed an increase for hybridoma cells cultured in whey medium. Glucose consumption rates were similar, whereas the lactate production rate was higher in whey medium. The metabolic uptake rates of glutamine and ammonia increased in whey medium. More alanine, glutamate, glycine, and proline were produced; their production partly came from glutamine and lactate. The consumption rates of branched amino acids changed little; their utilization was higher in whey medium. Finally, antibody productivity was increased about 20% for cells cultured in medium containing whey.

引用

页码：573 / 579

页数：7

共 50 条

[21] INTERMEDIATE FILAMENT PROTEINS IN MOUSE-BRAIN CELLS CULTURED IN THE PRESENCE OR ABSENCE OF FETAL CALF SERUM
PROCHIANTZ, A
DELACOURTE, A
DAGUET, MC
PAULIN, D
EXPERIMENTAL CELL RESEARCH, 1982, 139 (02) : 404 - 410
[22] Cerivastatin inhibits fetal calf serum-induced DNA synthesis in cultured rat mesangial cells
He, JS
Horikoshi, S
Funabiki, K
Shirato, I
Tomino, Y
NEPHROLOGY, 2002, 7 (02) : 83 - 86
[23] WHEY AND BETA-LACTOGLOBULIN .2. MILK BY-PRODUCTS WHICH CAN REPLACE FETAL CALF SERUM IN MOUSE HYBRIDOMA CELL-CULTURE
CAPIAUMONT, J
LEGRAND, C
DOUSSET, B
PARMENTELOT, I
LINDEN, G
BELLEVILLE, F
NABET, P
LAIT, 1994, 74 (02): : 127 - 137
[24] METABOLIC PROPERTIES OF HUMAN NEUROBLASTOMAS CULTURED IN SERUM SUPPLEMENTED AND DEFINED MEDIA
JOHN, N
BUSH, C
EBERT, C
TIMIRAS, PS
FEDERATION PROCEEDINGS, 1985, 44 (05) : 1386 - 1386
[25] Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
Monteiro Vianna, Lucas Monferrari
Li, Hao-Dong
Holiman, Jeffrey D.
Stoeger, Christopher
Belfort, Rubens, Jr.
Jun, Albert S.
ARQUIVOS BRASILEIROS DE OFTALMOLOGIA, 2016, 79 (01) : 37 - 41
[26] LONG-TERM CULTURE OF FETAL-RAT HEPATOCYTES IN MEDIA SUPPLEMENTED WITH FETAL CALF-SERUM ULTROSER-SF OR ULTROSER-G
YEOH, G
DOUGLAS, A
BRIGHTON, V
BIOLOGY OF THE CELL, 1986, 58 (01) : 53 - 63
[27] Comparison of methods to examine the endogenous peptides of fetal calf serum
Williams D.
Zhu P.
Bowden P.
Stacey C.
McDonell M.
Kowalski P.
Kowalski J.M.
Evans K.
Diamandis E.P.
Siu K.W.M.
Marshall J.
Clinical Proteomics, 2006, 2 (1-2) : 67 - 90
[28] Comparison of methods to examine the endogenous peptides of fetal calf serum
Williams, D.
Zhu, P.
Bowden, P.
Evans, K.
Marshall, J. G.
BIOPOLYMERS, 2007, 88 (04) : 607 - 607
[29] IMMUNOLOGICAL IDENTIFICATION OF FETAL CALF SERUM-DERIVED PROTEINS ON SURFACES OF CULTURED TRANSFORMED AND UNTRANSFORMED RAT CELLS
PHILLIPS, ER
PERDUE, JF
INTERNATIONAL JOURNAL OF CANCER, 1977, 20 (05) : 798 - 804
[30] HORMONAL-REGULATION OF HEMOGLOBIN SYNTHESIS IN CELLS OF FETAL CALF LIVER CULTURED IN A SERUM-FREE MEDIUM
CONGOTE, LF
SOLOMON, S
BIOCHEMISTRY, 1978, 17 (07) : 1160 - 1165

← 1 2 3 4 5 →