HIGH-LEVEL EXPRESSION OF FUSARIUM ALKALINE PROTEASE GENE IN ACREMONIUM-CHRYSOGENUM

被引：17

作者：

MORITA, S ^{[1
]}

KURIYAMA, M ^{[1
]}

NAKATSU, M ^{[1
]}

KITANO, K ^{[1
]}

机构：

[1] TAKEDA CHEM IND LTD, PROD RES LABS, DIV PHARMACEUT DEV, YODOGAWA KU, OSAKA 532, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 04期

关键词：

D O I：

10.1271/bbb.58.627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We transformed Acremonium chrysogenum with the genomic DNA of the alkaline protease (Alp) from Fusarium sp. S-19-5 including its promoter. Most of the transformants thus obtained produced a large amount of Alp. PCR and Southern hybridization analysis of genomic DNAs from these transformants showed chromosomal integration of the full-length Alp gene. SDS-PAGE analysis of the supernatant from the transformants showed the presence of Fusarium Alp. The amino terminus of the Alp produced in A. chrysogenum was identical to that of native Fusarium Alp. These results indicate that the Alp promoter, signal sequence, and introns functioned correctly in A. chrysogenum. One of the transformants produced more than 4g of the Alp per liter in a jar fermenter.

引用

页码：627 / 630

页数：4

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