DETERMINATION OF L-ALANINE IN A FLOW-INJECTION SYSTEM WITH AN IMMOBILIZED ENZYME REACTOR

被引：5
|
作者：
KIBA, N
TAGAMI, H
FURUSAWA, M
机构：
[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 239卷 / 02期
关键词：
Alanine; Beverages; Enzyme reactor; Serum;
D O I：
10.1016/S0003-2670(00)83867-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection system for the determination of l-alanine is described. Alanine dehydrogenase is immobilized on poly(vinyl alcohol) beads and used in a packed-bed enzyme reactor. The system responds linearly to injected samples (50 μl) in the concentration range 0.5-500 μM. The maximum throughput was 40 samples per hour. The immobilized enzyme reactor was stable for at least 6 weeks. Its usefulness for assay of l-alanine in serum and beverages is described. © 1990.
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页码：307 / 310
页数：4
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