IDENTICAL GENES CONFER HIGH-LEVEL RESISTANCE TO GENTAMICIN UPON ENTEROCOCCUS-FAECALIS, ENTEROCOCCUS-FAECIUM, AND STREPTOCOCCUS-AGALACTIAE

被引:31
|
作者
KAUFHOLD, A
PODBIELSKI, A
HORAUD, T
FERRIERI, P
机构
[1] RHEIN WESTFAL TH AACHEN,INST MED MIKROBIOL,W-5100 AACHEN,GERMANY
[2] INST PASTEUR,STAPHYLOCOQUES & STREPTOCOQUES LAB,F-75724 PARIS 15,FRANCE
[3] UNIV MINNESOTA,SCH MED,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN 55455
[4] UNIV MINNESOTA,SCH MED,DEPT PEDIAT,MINNEAPOLIS,MN 55455
关键词
D O I
10.1128/AAC.36.6.1215
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The structural gene coding for the bifunctional aminoglycoside-modifying 6'-acetyltransferase-2"-phospho-transferase (6'AAC-2"APH) enzyme was specifically amplified by the polymerase chain reaction using template DNA of clinical isolates of enterococci (both Enterococcus faecalis and Enterococcus faecium) and the single high-level gentamicin-resistant Streptococcus agalactiae strain identified thus far. The results of the present study demonstrated that the genes encoding this antibiotic resistance trait are highly homologous in these species. In dot blot hybridization assays using nonradioactively labeled oligonucleotide probes, strains with and without high-level gentamicin resistance could be discerned unequivocally. The gene segment encoding 6'-acetylating activity and the gene segment encoding 2"-phosphorylating activity were simultaneously present in all isolates exhibiting high-level gentamicin resistance.
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页码:1215 / 1218
页数:4
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