WATER-INSOLUBLE HIGH-MOLECULAR-WEIGHT AND ALPHA-CRYSTALLINS AS THE SOURCE OF THE SCHEIMPFLUG LIGHT-SCATTERING PATTERN IN THE RAT LENS

被引：4
|
作者：
BOURS, J
AHREND, MHJ
WEGENER, A
HOCKWIN, O
机构：
[1] Department of Experimental Ophthalmology, University of Bonn
来源：
OPHTHALMIC RESEARCH | 1990年 / 22卷
关键词：
Crystalline α- Pre-α- β- γ- High-molecular-weight Water-insoluble; Microsectioning technique; Rat lens; Scheimpflug Photography Densitometry;
D O I：
10.1159/000267074
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Lenses of 14-week-old rats were separated into 10 layers or fractions by a frozen-sectioning technique. The biochemical characteristics of these layers were assigned to corresponding parts of the densitometric reading obtained from the Scheimpflug negative, which enables a correlation of light scattering values recorded in vivo to protein patterns in the same area. Calculated as percentage of lens dry weight, all water-soluble crystalline show minima and the water-insoluble crystallins show maxima in the lens nucleus. This demonstrates that the nucleus contains the bulk of the water-insoluble high-molecular-weight and α-crystallins, being the source for the Scheimpflug light scattering pattern. eases (EURAGE). © 1990 S. Karger AG, Basel.
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页码：90 / 94
页数：5
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