Objective: To characterize the conditions of quality of working life, the presence of muscle-skeletal disorders and the association between these variables in nursing staff of a public hospital in Zacatecas, Mexico. Method: A cross-sectional study with descriptive-correlational scope was designed. A stratified random sampling per shift was used in 107 cases. The Questionnaire Professional Quality of Life( CVP-35) was applied as well as the Nordic Questionnaire Standardized for musculoskeletal pain and work-related risk factors questionnaire. Results: The quality of working life gained an average of 55.62 (SD = 13.57), the intrinsic motivation was the best rated component with (M = 75.06, SD = 18.44), contrary to managerial support that got the lowest scores with (M = 43.74, SD = 21.71). The presence of risk factors in the development work of musculoskeletal problems obtained a mean of 50.10 (SD = 26.69). The main musculoskeletal disorders occurred in the neck region, lumbar spine and knees with 42.1% for each one. The quality of working life decreased in the presence of muscle-skeletal problems in the lumbar region with (-0.188, p=.050), dorsal (-0.206, p=.050), neck (-0.175, p=.050) and knees (-0.220, p=.010). Conclusions: It is necessary to improve the working conditions of nurses to reduce the presence of musculoskeletal problems and improve their quality of working life. (C) 2016 Elsevier Espana, S.L.U. All rights reserved.