NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX PROTEIN PREVENTS MISTARGETING OF NASCENT CHAINS TO THE ENDOPLASMIC-RETICULUM

被引:100
|
作者
LAURING, B
SAKAI, H
KREIBICH, G
WIEDMANN, M
机构
[1] MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
[2] NYU, SCH MED, DEPT CELL BIOL, NEW YORK, NY 10016 USA
关键词
D O I
10.1073/pnas.92.12.5411
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We show that, after removal of the nascent polypeptide-associated complex (NAG) from ribosome-associated nascent chains, ribosomes synthesizing proteins lacking signal peptides are efficiently targeted to the endoplasmic reticulum (ER) membrane. After this mistargeting, translocation across the ER membrane occurs, albeit less efficiently than for a nascent secretory polypeptide, perhaps because the signal peptide is needed to catalyze the opening of the translocation pore. The mistargeting was prevented by the addition of purified NAC and was shown not to be mediated by the signal recognition particle and its receptor. Instead, it appears to be a consequence of the intrinsic affinity of ribosomes for membrane binding sites, since it can be blocked by competing ribosomes that rack associated nascent polypeptides, We propose that, when bound to a signalless ribosome-associated nascent polypeptide, NAC sterically blocks the site in the ribosome for membrane binding.
引用
收藏
页码:5411 / 5415
页数:5
相关论文
共 50 条
  • [1] NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX PROTEIN PREVENTS MISTARGETING OF NASCENT CHAINS TO THE ENDOPLASMIC-RETICULUM (VOL 92, PG 5411, 1995)
    LAURING, B
    SAKAI, H
    KREIBICH, G
    WIEDMANN, M
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) : 8088 - 8088
  • [2] Multifunctional nascent polypeptide-associated complex (NAC)
    Kogan, G. L.
    Gvozdev, V. A.
    MOLECULAR BIOLOGY, 2014, 48 (02) : 189 - 196
  • [3] Multifunctional nascent polypeptide-associated complex (NAC)
    G. L. Kogan
    V. A. Gvozdev
    Molecular Biology, 2014, 48 : 189 - 196
  • [4] Nascent polypeptide-associated complex stimulates protein import into yeast mitochondria
    Fünfschilling, U
    Rospert, S
    MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (10) : 3289 - 3299
  • [5] The nascent polypeptide-associated complex is essential for autophagic flux
    Guo, Bin
    Huang, Jie
    Wu, Wenxian
    Feng, Du
    Wang, Xiaochen
    Chen, Yingyu
    Zhang, Hong
    AUTOPHAGY, 2014, 10 (10) : 1738 - 1748
  • [6] Initial characterization of the nascent polypeptide-associated complex in yeast
    Reimann, B
    Bradsher, J
    Franke, J
    Hartmann, E
    Wiedmann, M
    Prehn, S
    Wiedmann, B
    YEAST, 1999, 15 (05) : 397 - 407
  • [7] Characterisation of the nascent polypeptide-associated complex in fission yeast
    Andersen, Katrine M.
    Semple, Colin A.
    Hartmann-Petersen, Rasmus
    MOLECULAR BIOLOGY REPORTS, 2007, 34 (04) : 275 - 281
  • [8] Structure and folding of nascent polypeptide chains during protein translocation in the endoplasmic reticulum
    Haynes, RL
    Zheng, TL
    Nicchitta, CV
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (27) : 17126 - 17133
  • [9] Characterisation of the nascent polypeptide-associated complex in fission yeast
    Katrine M. Andersen
    Colin A. Semple
    Rasmus Hartmann-Petersen
    Molecular Biology Reports, 2007, 34 : 275 - 281
  • [10] The nascent polypeptide-associated complex is a key regulator of proteostasis
    Kirstein-Miles, Janine
    Scior, Annika
    Deuerling, Elke
    Morimoto, Richard I.
    EMBO JOURNAL, 2013, 32 (10): : 1451 - 1468