SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES ON THE UPPER AND LOWER SURFACES OF THE FROG-SKIN EPIDERMAL-CELLS

We examined the fine structure of the upper and lower surfaces of stratified squamous epithelial cells in the skin of frogs (Hyla japonica). SEM revealed the upper surface of superficial cells covered with ramified microridges (type 3). The width of the microridges was 0.20-0.24-mu-m. Microridges found at the cell boundary were about 0.30-mu-m in width and a narrow furrow was seen between the two cells. The numerous oval disk-like structures (0.23 x 0.32-mu-m in diameter) covered the lower surface of these superficial cells. The upper surface of cells in the 2nd layer was covered with baculiform or ramified microridges (type 2 or 3). On the cell boundary, two linear microridges (0.23-0.27-mu-m in width) were parallelly arranged. The width of the microridges covering the upper cell surface was 0.09-0.10-mu-m. Microvilli-like processes with a height of 0.32-0.37-mu-m were interspersed among the microridges. Their tip formed an oval plane (0.23 x 0.31-mu-m), which corresponded to the size of the disk-like structures on the lower surface of the superficial cells. Desmosomes were observed on the tip by TEM. These findings show that the disk-like structures on the lower surface of the superficial cells are the sites of binding with the microvilli-like processes on the upper surface of the 2nd layer cells. The disk-like structures observed in the present study seem to be equivalent to the binding site on the upper surface of the surface cell layer of mammalian stratified squamous epithelium.