Alternative use of Gelred dye in the staining nucleic acids

Objetive: Determine the reproducibility of DNA staining in agarose gels using the dye GelRed (TM) in order to replace ethidium bromide in the use of nucleic acids staining. Materials and Methods: The extraction of the total DNA from nine samples and the amplified DNA fragment of 494 pb of exon 15 associated with the APC gene from six fragments between 150 pb - 350 pb associated with the hMLH-1 and MSH-2 genes was performed. Both preparations, total DNA and amplified DNA were visualized using GelRed and Ethidium bromide dye. Results: Both systems of coloration gave very similar results; the DNA can be visualized correctly without any difficulty. Conclusion: The GelRed dye is highly reproducible and its use is recommended including in DNA samples, thus reducing the contamination with dyes such as ethidium bromide.