OPTIMIZATION AND CONTROL IN FED-BATCH CULTURES

A method for obtaining maximum production of the bioproduct in fed-batch cultures is explained, and its validity is demonstrated by experimental data. The approach is based on a model which describes the relationship between the specific production rate, rho, and specific growth rate, mu. Using a mathematical model, an optimal profile of the specific growth rate could then be obtained easily by the Maximum Principle. Finally, the optimal profile was realized by changing the feed rate of the substrate in a practical fed-batch culture. Practical examples of bioproduction, such as histidine, lysine, and glutathione, as well as production of an enzyme, showed that the two-stage production process could be realized experimentally, thus demonstrating the validity of the method.