PARTIAL-PURIFICATION AND CHARACTERIZATION OF THE GIBBERELLIN-A20 3-BETA-HYDROXYLASE FROM SEEDS OF PHASEOLUS-VULGARIS

被引：29
|
作者：
SMITH, VA
GASKIN, P
MACMILLAN, J
机构：
[1] School of Chemistry, University of Bristol
来源：
PLANT PHYSIOLOGY | 1990年 / 94卷 / 03期
关键词：
D O I：
10.1104/pp.94.3.1390
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The GA20 3β-hydroxylase present in immature seeds of Phaseolus vulgaris has been partially purified and characterized. The physical characteristics of the enzyme are similar to those of the GA 2β-hydroxylases present in mature and immature seeds of Pisum sativum. It is acid-labile, hydrophobic, and of Mr 45,000. The enzyme catalyzes the synthesis of GA1, GA5, and GA29 from GA20. Activity is dependent upon the presence of Fe2+, ascorbate, 2-oxoglutarate, and oxygen. 2-Oxoglutarate does not function as a cosubstrate; in the presence of the enzyme, succinate is not a reaction product.
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页码：1390 / 1401
页数：12
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